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KMID : 1007520150240041475
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Food Science and Biotechnology
2015 Volume.24 No. 4 p.1475 ~ p.1480
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Kaempferol Isolated from Nelumbo nucifera Stamens Inhibits Phosphorylation of ERK 1/2, Syk, and Lyn in FcεRI-mediated Allergic Reaction
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Shim Sun-Yup
Park Jeong-Ro
Byun Dae-Seok
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Abstract
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Cross-linking of FcεRI with multivalent allergen and specific IgE on mast cells and initiates activation of signal cascades that lead to IgE-mediated allergic responses. Kaempferol is a phenolic compound contained in natural substances including the stamens of flavonoid-rich Nelumbo nucifera stamens. In this study, the effects of kaempferol on allergic reaction induced by an FcεRI α chain antibody were investigated. Western blot analysis revealed that the protein expression of FcεRI and the phosphorylation of Syk, Lyn, and extracellular signal-regulated kinases (ERK) 1/2 were dose-dependently inhibited by kaempferol. β- Hexosaminidase was reduced to 97.9, 89.1, 75.1, and 55.8% when treated with 5, 10, 20, and 40 μΜ kaempferol, respectively. From the results, the negative regulation of basophil activation with kaempferol via the suppression of FcεRI expression and degranulation may be suggested to be through inhibition of Lyn and Syk, and ERK 1/2 activation.
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KEYWORD
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Nelumbo nucifera stamens, kaempferol, Syk, Lyn, ERK 1/2
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